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    <title>fluorescent tags | Rudolf Schlechter</title>
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      <title>fluorescent tags</title>
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      <title>Synthetic Biology</title>
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      <pubDate>Thu, 26 May 2022 13:20:10 +0200</pubDate>
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      <description>&lt;p&gt;Bacterial individuality can only be acknowledge at the single-cell level. To observe bacterial cells, we have developed a series of plasmids to tag them with unique fluorescent proteins. These plasmids are based on an expression, Tn5- and Tn7-delivery vectors. Check them out &lt;a href = &#34;https://www.frontiersin.org/articles/10.3389/fmicb.2018.03052/full&#34; target=&#34;_blank&#34;&gt;here!&lt;/a&gt;. If you&amp;rsquo;re interested in them, they&amp;rsquo;re also available on &lt;a href = &#34;https://www.addgene.org/browse/article/28196767/&#34; target=&#34;_blank&#34;&gt;Addgene.org&lt;/a&gt;&lt;/p&gt;
&lt;p&gt;We keep improving on our abilities to modify non-model microorganisms through new methods and tools. I&amp;rsquo;m currently working on new tools based on a CRISPR system and different transposase genes.&lt;/p&gt;
&lt;p&gt;Also, I&amp;rsquo;m involved in the development of bioreporters that would inform us on bacterial activity and physiology in the phyllosphere at the micrometre resolution.&lt;/p&gt;
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